Explore methods developed by and/or used in VHP4Safety.
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The Virtual Human Platform implements a generic safety assessment as a five-step process. For more information regarding the Safety Assessment Workflow, visit our Safety Assessment Workflow info page.
Fluorescence-based determination of cell membrane damage
Fluorescence-based detection of mitochondrial metabolic activity
A Non-Lytic Fluorescence Assay That Measures the Relative Number of Viable Cells in Culture
ROS detection by fluorescent probe
Luminescence-based detection of cellular ATP content
Fluorescence-based detection of LDH
Fluorescence-based detection of reactive oxygen species production
immunofluorescent detection of collagen - 1
Intracellular measurements are determined in cell lysates using LCMS, normalized by protein expression that has been measured by 2D Quant
KIM-1 staining is an immunofluorescent staining of kidney injury molecule 1, an protein that is expressed when kidney injury takes place
Detection of L-lactate in biological samples
Colorimetric determination of lactate dehydrogenase (LDH) activity
Fluorescent - based detection of lysosomes
Immunofluorescent staining of P-gp protein, the efflux transporter of tacrolimus
mitochondrial membrane potential assay
Fluorescent-based assay to label mitochondria
Technology used for measuring RNA using PCR
Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells
next-generation sequencing technique used to quantify and identify RNA molecules in a biological sample
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