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Methods

Explore methods developed by and/or used in VHP4Safety.

You can search for a method that you know using the search box below. Alternatively, you can filter the methods with respect to the case studies' flow step that they belong to and/or the regulatory question that they are associated with. Use the "Filters" button to show filter options, then "OK" to see results, and click "X" to reset the filters.

    H2DCFDA-AM assay for detection of oxidative stress production

    Fluorescence-based detection of reactive oxygen species production

    Collagen I Polyclonal Antibody

    immunofluorescent detection of collagen - 1

    Intracellular measurements

    Intracellular measurements are determined in cell lysates using LCMS, normalized by protein expression that has been measured by 2D Quant

    KIM-1 staining

    KIM-1 staining is an immunofluorescent staining of kidney injury molecule 1, an protein that is expressed when kidney injury takes place

    Lactate assay

    Detection of L-lactate in biological samples

    LDH activity assay to determine cell death

    Colorimetric determination of lactate dehydrogenase (LDH) activity