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Inhibition of OATP1C1-mediated cellular uptake of thyroxine

A cell based in vitro assay to screen chemicals for their capacity to inhibit thyroxine (T4) uptake by human organic anion transporter polypeptide 1C1 (OATP1C1) using an overexpressing cell model.

Details
Type
study
Internal ID
S-VHPS26
Release date
2026-03-18
Version
—
License
CC0
URL
https://www.ebi.ac.uk/biostudies/studies/S-VHPS26
DOI
10.6019/S-VHPS26
Files

No files listed.

Authors
Funding
  • EU Horizon 2020
    82516
CHO-K1 cells
RRID: CVCL_0214
Supplier: ECACC · Cricetulus griseus (Chinese hamster) , Homo sapiens (human) · Ovary · Epithelial ovary cell
Cell name: CHO-K1 OATP1C1
Cell type: immortalized cell line
Genetic modification: Chinese hamster ovary (CHO-K1) cells stably transfected with a pCRII-TOPO vector containing a 3.1 kb fragment encoding for human OATP1C1.
Reference source: https://doi.org/10.1210/me.2001-0309
Silychristin
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Lesinurad
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Indocyanine green
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Verapamil
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Diclofenac
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Probenecid
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Bromosulfophthalein
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Bisphenol-S
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Bisphenol-Z
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Bisphenol-AF
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Bisphenol-F
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Sulforhodamine 101
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Pentachlorophenol
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Tetrabromobisphenol-A
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Bisphenol-A
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Perfluorooctanesulfonic acid
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Perfluorooctanoic acid
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Quercetin
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Rifampicin
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Triclosan
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Single exposure
Duration: 30 minutes
Time between end of exposure and start measurement: 20 · Time unit: minutes
Bioassay
Uptake transporter inhibition assay BAO
Wizard2 gamma counter
Manufacturer: Perkin Elmer
Measured: Radioactivity · Readout: T4 uptake (percentage of total T4 uptake) · Replicates: 3 to 4
Measured entity_unit: counts per minute
AOP Linkage

Coming soon: normalization, model fitting, statistics, QC, etc.

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