Inhibition of OATP1C1-mediated cellular uptake of thyroxine

A cell based in vitro assay to screen chemicals for their capacity to inhibit thyroxine (T4) uptake by human organic anion transporter polypeptide 1C1 (OATP1C1) using an overexpressing cell model.

Details

Type: study
Internal ID: S-VHPS26
Release date: 2026-03-18
Version: —
License: CC-BY
URL: https://www.ebi.ac.uk/biostudies/studies/S-VHPS26
DOI: 10.6019/S-VHPS26

Publications

Loading citation…

https://doi.org/10.1007/s00204-024-03787-2

Files

No files listed.

Authors

Fabian Wagenaars
Universiteit Utrecht
0000-0003-4766-7358

Funding

EU Horizon 2020
825161

CHO-K1

RRID: CVCL_0214 · Supplier: ECACC

Cricetulus griseus (Chinese hamster) , Homo sapiens · Ovary · Epithelial ovary cell · Immortal female gonad-derived cell

Silychristin A

CAS: 33889-69-9
PubChem: CID 441764
CompoundCloud: Q1881

Lesinurad

CAS: 878672-00-5
PubChem: CID 53465279
CompoundCloud: Q1880

Indocyanine green

CAS: 3599-32-4
PubChem: CID 5282412
CompoundCloud: [DRAFT]

Verapamil Hydrochloride

CAS: 152-11-4
PubChem: CID 62969
CompoundCloud: [DRAFT]

Diclofenac sodium

CAS: 15307-79-6
PubChem: CID 5018304
CompoundCloud: [DRAFT]

Probenecid

CAS: 57-66-9
PubChem: CID 4911
CompoundCloud: [DRAFT]

Sulfobromophthalein sodium

CAS: 71-67-0
PubChem: CID 6282
CompoundCloud: [DRAFT]

Bisphenol-S

CAS: 80-09-1
PubChem: CID 6626
CompoundCloud: Q81

Bisphenol-Z

CAS: 843-55-0
PubChem: CID 232446
CompoundCloud: Q96

Bisphenol AF

CAS: 1478-61-1
PubChem: CID 73864
CompoundCloud: Q89

Bisphenol-F

CAS: 620-92-8
PubChem: CID 12111
CompoundCloud: Q90

Sulforhodamine 101

CAS: 60311-02-6
PubChem: CID 122180
CompoundCloud: [DRAFT]

Pentachlorophenol

CAS: 87-86-5
PubChem: CID 992
CompoundCloud: Q4788

Tetrabromobisphenol-A

CAS: 79-94-7
PubChem: CID 6618
CompoundCloud: Q82

Bisphenol-A

CAS: 80-05-7
PubChem: CID 6623
CompoundCloud: Q80

Perfluorooctanesulfonic acid

CAS: 1763-23-1
PubChem: CID 74483
CompoundCloud: Q2373

Perfluorooctanoic acid

CAS: 335-67-1
PubChem: CID 9554
CompoundCloud: Q2368

Quercetin

CAS: 117-39-5
PubChem: CID 5280343
CompoundCloud: [DRAFT]

Rifampicin

CAS: 13292-46-1
PubChem: CID 135398735
CompoundCloud: Q37

Triclosan

CAS: 3380-34-5
PubChem: CID 5564
CompoundCloud: Q4790

Single exposure

Duration: 30 minutes

Bioassays: CHO-K1 OATP1C1 TH uptake, CHO-K1 OATP1C1 cell viability

Exposure-measurement interval: 0 minutes · Exposure concentration: [DRAFT] µM

Bioassays

CHO-K1 OATP1C1 TH uptake BAO

Inhibition, organic anion-transporting polypeptide 1C1 (OATP1C1)

Measured: Radioactivity · Readout: T4 uptake (percentage of total T3 uptake) · Replicates: 3-4 · Technical replicates: 3 · Positive control: Indocyanine green (100)
CHO-K1 OATP1C1 cell viability BAO

Measured: Luminescence · Readout: Cell viability (ATP) (BMC10) · Replicates: 2 · Technical replicates: 3 · Positive control: DMSO (10)

AOP Linkage

Coming soon: normalization, model fitting, statistics, QC, etc.

Loading dataset citation…

Thyroid Question 2 Toxicodynamics

Menu